Methods and Techniques
Electrophysiology experiments involve intracellular recordings of the neural activity from spinal motoneurons under healthy and disease conditions from in-vitro preparations (patch-clamp in slices and sharp-electrode recordings in the whole cord preparation).
Immunohistological staining of the motoneuron allows us to examine the expression of ion channels on the cell membrane of motoneurons.
We are intertesed in developing computer models of spinal motoenruons, based on their 3D anatomy, to examine their firing behaviors.
We examine behavioral symptoms and monitor the survival of animals with motoneuron disease in order to determine functional changes using different treatment options.